The neurosphere assay for developmental neurotoxicity testing

analogous threshold rules applied, followed by expert intervention for data points for which the automated system failed to generate an expert call. The semi-automated expert calls, which were completed in 5 days, compared to 3 weeks for the manual approach, agreed with the human expert calls for 93% of the 6956 chemicals based on a conservative approach for all combined classified calls. As a proof of concept, the complex nuclear receptor binding/ activation data was successfully transformed into a purposeful dataset. This methodology can be extended to other MIEs, including enzyme inhibition. MIE datasets created this way have useful applications, including faster structural alerts development in a transparent in silico system like Derek Nexus, for insertion in a purposeful database, to support adverse outcome pathway frameworks and for model building.